All NeuroMabs are mouse monoclonal antibodies generated from hybridoma cells grown either in vitro or in vivo. The different forms are:

TC supernatant: conditioned bovine serum-containing media harvested from hybridomas cultured in vitro. Typically contains 10-50 micrograms/ml mouse monoclonal IgG plus bovine IgG and other bovine serum components.

Recommended working dilutions for TC supernatants are 1:10-1:100 for immunoperoxidase-based immunohistochemistry, and 1:2-1:10 for immunofluorescence-based immunohistochemistry and immunocytochemistry, and immunoblotting. These recommended working dilutions are meant to be a starting point for optimization based on your specific application.

Purified IgG: Purified from hybridoma cells grown in vivo. Predominantly monoclonal IgG but may contain trace amounts of other mouse IgG.

Recommended working concentrations for purified IgGs are 0.1-1 ug/ml for immunoperoxidase-based immunohistochemistry, and 1-10 ug/ml for immunofluorescence-based immunohistochemistry and immunocytochemistry, and immunoblotting. These recommended working concentrations are meant to be a starting point for optimization based on your specific application.

A useful reference for this and related information is Harlow, E. and Lane, D. 1988. Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory Press